Police were at the scene on Monday to investigate an attempted burglary where it is believed two men broke into the station just after 6am.

It is believed the men forced entry to the station through an entrance door before smashing a counter window.

The train station remained closed on Monday as police investigated the scene.

Police say the men left empty handed and that the Myki machine was not damaged.

Police tape cordoned off the station-side entrance where it appeared the door lock had been damaged.

The station remained closed on Monday and the station master said he could not make any comment on the incident.

V/Line spokesperson Ebony Jordan confirmed a break-in had occurred at the station on Monday morning, however said it was not known when the station would reopen.

"V/Line is assisting police with their investigations following a break in at the Ballan Station in the early hours of this morning," Ms Jordan said.

"We will work with the police to determine when the station reopens." 

Moorabool Police Crime Investigation Unit are urging any witnesses to contact Bacchus Marsh police station on 5366 4500 or Crime Stoppers on 1800 333 000.