For the last 15 months cyclists say a rogue individual has targeted them by putting thumb tacks along the shoulder of the road, causing punctured tyres and crashes.

VicRoads had swept the popular cycle route at 11pm on Saturday night to clear it of tacks, but the so-called "boulie tacker" was at it again.

"There's clearly been a fresh batch of tacks, which is quite brazen really," protest organiser George Mihailides said.

Efforts to fix the problem have come to naught and now  cyclists are calling for surveillance cameras to be installed along the road to catch the culprit.

Mr Mihailides said the problem was forcing cyclists away from the shoulder of the road and into the traffic lane, endangering them and motorists.

The problem was causing "crashes, injuries, motorists getting punctured, motorcyclists getting punctured, injured children, injured pets and let's not forget the hundreds of thousands of dollars this is costing the ratepayers of Boroondara", he said.

"It's only a matter of time before someone gets seriously injured, or worse."

Victoria Police is investigating the matter. A spokesperson said around 500 cyclists had suffered punctures in the last  year.

The clean-up cost had amounted to around $70,000 and the repair costs to bike riders around $5000 so far, the police spokesperson said.

The main problem areas were at the intersections of Walmer Street, Studley Park Road overpass, Molesworth Street, Yarra Park Bend and the intersection of Witshire Drive and Chandler Highway, they said.

Got me again on this mornings #boulietacks ride. That makes 13 since it started pic.twitter.com/VVRCz8E7NL— Wendy Osland (@wendypauline) May 3, 2015

Mr Mihailides said police, VicRoads, the local council and Parks Victoria needed to work together to solve solve the problem.

"If you ask any of the bodies involved they all try to say it's not our piece of road to manage, so we're looking for united leadership," Mr Mihailides said. "We're not quite sure who it should be but someone needs to own it as a problem."

Spokespeople for Parks Victoria and Boroondara Council said separately that large roads were VicRoads' jurisdiction.

A VicRoads spokesperson was not available for comment.

With support from Borondoora Council and local bike shops, the community has set up "puncture stations" at intervals along the route.

Cyclists can use a spare tube if they get a puncture, and replace it later, under an honour system. But sometimes the tubes are stolen.

Some fools be breaking things down, but the better peeps be building them up #boulietacks. pic.twitter.com/BZMvYdZ3Nb— Stuart Partridge (@stuartp) May 1, 2015

A Parks Victoria manager Philip Ross said it did not manage the Yarra Boulevard and therefore had no direct authority over it.

"We've spoken this week to the protest organiser and explained we do not manage the Boulevard," he said. "We are happy to work with Boroondara Council and Victoria Police if there is any way we can assist within our areas of responsibility."