The Andrews government has long claimed it is removing "Melbourne's 50 most dangerous and congested level crossings" and is spending more than $20 million of taxpayers' money to spruik the project.

It will use the bulk of the proceeds from its $9.7 billion long-term lease of the Port of Melbourne to pay for its signature eight-year removal program, which has already seen 10 crossings removed and eight railway stations torn down and rebuilt.

Data from VicRoads, the Department of Transport and the City of Wyndham reveals some of the crossings on Labor's hit list are considered far less dangerous and congested than many that will be left in place indefinitely.

For example, at the Werribee Street level crossing, boom gates drop for just five minutes in the two-hour morning peak, and for just 90 seconds in the afternoon peak.

Planning for its removal is already under way, even though it is crossed by just a few freight trains each day.

Meanwhile 15 of the 50 crossings on Labor's list were accorded no priority in a 2014 VicRoads report that prioritised the city's approximately 170 level crossings for removal based on their impact on congestion and safety.

The Coalition has used this data to argue Labor is therefore wasting scarce public funds on deliberately misleading self-promotion.

It has asked the Victorian Auditor-General to intervene and investigate if the government has breached state communications guidelines.

The guidelines state that with all taxpayer-funded advertising, "facts, statistics and comparisons must be presented accurately and objectively, to ensure that information can be substantiated".

"The repeatedly advertised claim of the Andrews government that 50 of our 'most dangerous and congested level crossings' are being removed is clearly false and misleading," shadow public transport minister David Davis said.

"Worse, it is designed to hoodwink the very consumers who are footing the bill."

Crossings scheduled for removal that VicRoads ranked as low-priority include Kororoit Creek Road in Williamstown North, Camp Road in Campbellfield and Abbotts Road in Dandenong South, all three of which are already being removed.

Another is Grange Road in Alphington, which Premier Daniel Andrews visited on Friday ahead of a nine-day shutdown for works.

Mr Andrews dismissed the Coalition's accusation. He said he would not be lectured to by a party that had removed very few level crossings when it was in government.

"We know, as do Victorians, that these level crossings are deadly, they're congested, they're stopping us from running more trains more often - they're relics of the past and that's where we need to consign them to," Mr Andrews said.

The Hurstbridge line will be closed between Clifton Hill and Greensborough for nine days from 9pm Friday, with buses replacing trains, while work to remove the Grange Road level crossing ramps up.

Grange Road, a major north-south truck route in Melbourne's inner north-east, will be shut at the level crossing from 9pm Friday until Tuesday morning.