Victoria has recorded a 12th consecutive day without a local coronavirus case, following a scare among staff at a quarantine hotel.
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The Department of Health and Human Services on Monday confirmed there were four new COVID-19 cases in hotel quarantine, bringing the total number of active cases in the state to 33.
Almost 14,000 tests were processed in the past 24 hours.
It comes after a nurse and a police officer working at a medi-hotel in Melbourne's CBD tested negative to the virus after initially returning "weak positive" results.
The positive saliva swabs led to 41 other workers at the Holiday Inn on Flinders Street being stood down.
"Saliva tests are very reliable with negative results, but can return occasional false positives," a spokesman from COVID-19 Quarantine Victoria said.
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The hotel is being used to quarantine COVID-positive returning travellers.
Meanwhile, the Australian Open has been hit by another setback as a fifth person tested positive for coronavirus, forcing 25 more players into strict quarantine.
A total of 72 players are now isolating in their hotel rooms ahead of the tournament.
The fifth person tested positive on Sunday night and they were on a charter flight from Doha that landed in Melbourne on Saturday.
It means three Australian Open charter flights have carried passengers who later tested positive, following infections on planes from Los Angeles and Abu Dhabi.
COVID-19 Quarantine Victoria on Sunday said four people in the Australian Open contingent and one airline crew member had now tested positive.
Despite some of the affected players complaining of being unable to leave their rooms for training, COVID-19 Quarantine Victoria commissioner Emma Cassar insisted the program would not be modified.
Ms Cassar said there had been cases of "challenging behaviour" from some confined players and support staff.
She cited two cases when they opened their doors to have a conversation or shout down the hallway.
"There is zero tolerance for breaches," she said.
"I had a conversation with Victoria Police to ensure we are increasing our compliance and enforcement efforts.
"It's low level but dangerous acts that we just can't tolerate."
Ms Cassar warned they could be fined up to $20,000, or repeat offenders transferred to the complex care hotel where they have a police officer stationed outside their door.
Australian Open boss Craig Tiley has ruled out delaying the tournament, which is due to begin on February 8.
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