Victoria has gone five days without recording a coronavirus case.
The Health Department confirmed there were no new local or overseas acquired cases recorded on Wednesday, following 20,201 tests.
There remain 20 active cases of COVID-19 in the state, a decrease in five since Tuesday.
Thousands, however, remain in self-isolation after coming into contact with cases linked to the Holiday Inn outbreak.
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A total of 22 cases have been traced back to a family of three staying on the third floor of the Melbourne Airport hotel in early February, who contracted the highly-infectious UK strain of the virus overseas.
Fears the virus would spread into the community led to a five-day "circuit- breaker" lockdown, which ended last Thursday.
Some restrictions, including mask-wearing and strict gathering limits, remain until Friday when the last close contacts end 14 days of isolation.
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