Neuron, the company running the trial - a joint state government and City of Ballarat initiative - will hold a safety event on Thursday from 11.30am at the Town Hall.

Participants will take a short safety briefing and can earn "rewards such as free credits for future rides", according to a Neuron media release.

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The company has also released a number of safety videos to remind people of the rules.

The sessions will be in partnership with Victoria Police, which has the power to issue fines for infringements.

E-scooter riders must be over 18, and as part of the trial, must not ride on footpaths or in areas where the speed limit is over 50km/h.

Helmets must be worn at all times, and riders risk losing their drivers licence or learners permit if using an e-scooter under the influence of alcohol.

Riding on the footpath faces a fine of $182, riding without a helmet will result in a $227 fine and riding while under the influence of alcohol, faces a fine of $227 plus disqualification.

Ballarat Highway Patrol's senior sergeant Stuart Gale said police will definitely be out and about.

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"We'll certainly be conducting more checks - what we've seen so far is that compliance is waning," he said, amid anecdotal evidence of helmets not being worn and e-scooter use on 60km/h streets such as Creswick Road.

Last week, 250 shared e-scooters were distributed across the CBD, with several areas geo-fenced, or automatically locked-out.

According to Neuron, the average e-scooter trip is about 2.8km, with Town Hall being a popular place to start and end a ride.

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Lake Wendouree, where e-scooters are automatically limited to 15km/h and with fewer parking areas, has also seen many trips, the company added.

Neuron was not able to confirm the exact number of trips taken so far.

Neuron's Australia and New Zealand head, Richard Hannah, urged people to take advantage of the safety session on Thursday, particularly first-time e-scooter riders.

"It is great to be partnering with Ballarat Police to help promote e-scooter safety," he said in a statement.

"With thousands of trips taken in the first few days alone, we are already seeing how popular our e-scooters are in the city. We want to take this opportunity to reinforce the rules as well as our safety guidelines - it is the responsibility of every rider to do the right thing.

"Ultimately, we're committed to providing a safe, convenient and environmentally-friendly service for our users but also for the general public."

In a statement, City of Ballarat mayor Daniel Moloney said council is "delighted" to see so many residents and visitors trying out the e-scooters.

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"While the early uptake and feedback is overwhelmingly positive, we do want to remind people to follow the riding rules," he said.

"Especially with school holidays now here, we want to remind the community that while we know people of all ages will be tempted to ride, it is important to remember you must be over 18."

Private e-scooters can only be ridden in public with a speed limit of 10km/h and a motor of 200 watts, riding one outside of these specifications on public roads will attract a fine of $826.

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