Emergency crews were called to a two-car collision at the intersection of Creswick Road and Howitt Street, Wendouree.
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The alert went out about 8.40pm on Thursday evening, with several ambulances attending.
Both cars appeared extensively damaged, with reports from witnesses that there were several people involved.
An Ambulance Victoria spokesperson said one woman in her 50s was taken to Ballarat Base Hospital with upper body pain.
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