Victoria has recorded no new locally acquired coronavirus cases, ahead of a further easing of restrictions across Melbourne and the regions on Friday.
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The Health Department on Thursday confirmed just one new COVID-19 case involving a returned traveller in hotel quarantine.
The total number of active cases in the state is now 54.
Some 25,635 Victorians were tested in the 24 hours to midnight on Wednesday while 15,610 received a COVID-19 vaccine dose at state-run hubs.
It comes ahead of an easing of restrictions in Melbourne and regional Victoria from Friday.
Under the latest changes, Melbourne residents will be able to travel to regional Victoria with the removal of the 25km journey bubble, host two adult visitors plus their dependents per day and gather outdoors in groups of 20.
Masks will remain mandatory indoors but will only be required outdoors when social distancing isn't possible.
Businesses such as gyms and indoor entertainment venues will be able to reopen, while density limits at offices, cafes, restaurants and pubs will increase.
In regional Victoria, the home visit cap will increase to five adults plus their dependents per day, while up to 50 people can gather outdoors.
Acting Premier James Merlino says restrictions could ease further in a week's time if COVID-19 cases remain low.
"We have to ease in a way that is safe, and we are making positive progress," he said on Wednesday.
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Chief Health Officer Brett Sutton said there still may be undetected cases in the community and urged people not to slacken off on following rules.
"It's really tough. This is 16 months into a pandemic. There is no question that people are over this," he said.
Professor Sutton said authorities would have to balance the ongoing need for masks in high-risk indoor settings over winter against fatigue.
"We don't want people to be fatigued by mask-wearing and basically not doing it," he said.
Victoria recorded five new local COVID-19 cases in the 24 hours to Wednesday morning, including two cases linked to a Southbank townhouse complex announced on Tuesday.
Two of the three other new cases are close contacts of people within the Kings Park Apartment Complex who previously tested positive for the virus.
It brings the total number of cases at the complex to eight, including an infant.
The other new case is a nurse at Epping Private Hospital, where three COVID- positive Arcare Maidstone aged care residents were previously transferred.
COVID-19 commander Jeroen Weimar said the nurse had received at least her first dose of the vaccine and may have been infectious during at least one of two shifts this month.
The woman also worked at a medical ward at the Northern Hospital on June 11 and 12.
More than a dozen new exposure sites have been listed across Brunswick, Pascoe Vale and the Crown Casino complex.
Anyone who watched the movie Cruella at Crown's Village Cinemas on June 12 at 7.30pm has been told to quarantine for 14 days.
Meanwhile, the state government has ramped up the range of experts who will be on hand to give advice about its planned mRNA vaccine manufacturing facility.
US immunologist and virologist Dr Barney Graham, whose ground-breaking research on mRNA helped lead to the development of the Moderna vaccine, will join the nine-person advisory group.
The technology of mRNA teaches cells how to make a protein to trigger an immune response.
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